surfactant protein d  (R&D Systems)

Journal: Molecular Medicine Reports

Article Title: Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air-blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF-κB/IκBα pathway

doi: 10.3892/mmr.2026.13797

Figure Lengend Snippet: Injury of the air-blood barrier induced in all groups. (A) The expression level of ZO-1 and claudin-5 in the lung tissue of rats was tested using immunohistochemistry (×200 magnification). The values are expressed as mean ± SE; (n=8). **P<0.01 vs. normal group. (B) Protein expression of ZO-1, claudin-5, SP-D, HO-1 and Ang-2 in the lung tissue tested via western blotting. The values are expressed as mean ± SE; (n=3). *P<0.05 and **P<0.01 vs. normal group. (C) The expression level of AQP-5, SP-D and CD31 in the lung tissue tested using immunofluorescence (×200 magnification). ZO-1, zonula occludens-1; SP-D, surfactant protein-D; HO-1, heme oxygenase-1; Ang-2, angiopoietin 2; AQP-5, aquaporin-5; IOD, integrated optical density.

Article Snippet: The slices were permeabilized with 0.2% Triton X-100, followed by blocking in 3% BSA for 1 h. The slices were incubated with target-specific primary antibodies overnight at 4°C: aquaporin (AQP)-5 (1:1,000; cat. no. 20334-1-AP; Proteintech Group, Inc.), surfactant protein-D (SP-D; 1:1,000; cat. no. DF13601; Affinity Biosciences), CD31 (1:1,000; cat. no. AF6191; Affinity Biosciences).

Techniques: Expressing, Immunohistochemistry, Western Blot, Immunofluorescence

Journal: Molecular Medicine

Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release

doi: 10.1186/s10020-025-01228-z

Figure Lengend Snippet: An Increase in Circulating mtDNA Is Associated with Inflammation and Necroptosis in Septic ALI. ( A ) Representative H&E-stained photomicrographs of murine lung tissues from the control (0 h), 6 h, 12 h, and 24 h groups. Bar = 25 μm (original magnification ×100, ×400). ( B ) Lung injury scores for the control (0 h), 6 h, 12 h, and 24 h groups. ( C ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) measured by ELISA in mice after CLP at 0, 6, 12, and 24 h. ( D-I ) Correlations between serum mtDNA levels and lung injury biomarkers (SP-D and sRAGE) assessed using the Spearman correlation test. mtDNA levels were analyzed via quantitative real-time PCR using three mtDNA primers (D-loop, mt-COX1, mMito). ( J ) Western blot analysis of necroptosis signaling in murine lung tissues at 0, 6, 12, and 24 h after CLP. ( K ) Quantification of Western blot results. Data are presented as mean ± SD from at least four independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

Article Snippet: Mouse Surfactant protein D ELISA KIT , Solarbio , SEKM-0196.

Techniques: Staining, Control, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot

Journal: Molecular Medicine

Article Title: Melatonin alleviates sepsis-induced acute lung injury by inhibiting necroptosis via reducing circulating mtDNA release

doi: 10.1186/s10020-025-01228-z

Figure Lengend Snippet: Melatonin Mitigates Septic ALI by Reducing Circulating mtDNA Release and Inflammatory Injury. ( A ) Survival rates of mice with or without melatonin therapy 24 h after CLP (log-rank [Mantel-Cox] test, n = 20). ( B ) Lung wet/dry ratio in mice with or without melatonin therapy at 24 h after CLP. ( C ) BALF protein levels in mice with or without melatonin therapy at 24 h after CLP. ( D ) Representative H&E-stained photomicrographs of lung tissues from Sham, Sham + Mel, CLP, and CLP + Mel groups. Bar = 25 μm (original magnification ×100, ×400). ( E ) Lung injury scores from the Sham, Sham + Mel, CLP, and CLP + Mel groups. ( F-J ) Plasma levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in Sham, Sham + Mel, CLP, and CLP + Mel groups. ( K-O ) BALF levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, MIP-2, and HMGB1) measured by ELISA in the same groups. ( P-R ) qPCR analysis of mt-Col, mt-Dloop, and mMito mRNA levels in indicated tissues. Data are presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. One-way ANOVA followed by Tukey’s multiple comparisons test

Article Snippet: Mouse Surfactant protein D ELISA KIT , Solarbio , SEKM-0196.

Techniques: Staining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay